EC Number   |
Natural Substrates |
|---|
   2.5.1.20 | cis-farnesyl diphosphate + isopentenyl diphosphate |
the enzyme is involved in the production of natural rubber. It catalyzes the sequential cis-1,4-condensation of isopentenyl diphosphate with cis-farnesyl diphosphate and its growing chains |
| 2.5.1.20 | isopentenyl diphosphate + cis-1,4-polyisoprene |
incorporation of isopentenyl diphosphate into rubber |
| 2.5.1.20 | isopentenyl diphosphate + cis-1,4-polyisoprene |
50 mM phosphate buffer, pH 7.5, 40 mM 2-mercaptoethanol, 40 mM KF, 8 mM MgCl2 + substrates, 30°C, 70% incorporation small rubber particles, 50% incorporation with FL-latex, 13% with washed small particles, 4% with highly purified washed small particles, with farnesyl diphosphate or dimethylallyl diphosphate as allylic initiators 70% incorporation, similar as for small rubber particles without allylic initiators |
| 2.5.1.20 | more |
natural rubber is synthesized by adding activated 2-methyl-1,3-butadiene to the growing chain |
| 2.5.1.20 | more |
presence of a single rubber transferase capable of using isopentenyl diphosphate in purified rubber particles |
| 2.5.1.20 | polycis-polyprenyl diphosphate + isopentenyl diphosphate |
- |
| 2.5.1.20 | polycis-polyprenyl diphosphate + isopentenyl diphosphate |
the enzyme is involved in the production of natural rubber. It catalyzes the sequential cis-1,4-condensation of isopentenyl diphosphate with cis-farnesyl diphosphate and its growing chains |
