EC Number   |
Natural Substrates |
|---|
   2.5.1.148 | 2 (2E,6E)-farnesyl diphosphate + NADPH + H+ |
- |
| 2.5.1.148 | 2 geranylgeranyl diphosphate + NADPH + H+ |
LOS catalyzes the condensation of two GGPP units in the first half reaction to form the cyclopropylcarbinyl diphosphate intermediate PLPP, with concomitant release of one molecule of inorganic diphosphate. In the second half reaction, the PLPP cyclopropyl ring is cleaved and rearranged to form a 1-1' linkage and further reduction by NADPH forms lycopaoctaene. Without NADPH only the reaction intermediate PLPP accumulates |
| 2.5.1.148 | 2 geranylgeranyl diphosphate + NADPH + H+ |
the enzyme is involved in biosynthesis of (14E,18E)-lycopadiene overall reaction. Lycopaoctaene i.e. 15,15'-dihydrophytoene = (6E,10E,14E,18E,22E,26E)-2,6,10,14,19,23,27,31-octamethyldotriaconta-2,6,10,14,18,22,26,30-octaene |
| 2.5.1.148 | 2 geranylgeranyl diphosphate + NADPH + H+ |
the enzyme is involved in biosynthesis of (14E,18E)-lycopadiene. Lycopaoctaene i.e. 15,15'-dihydrophytoene = (6E,10E,14E,18E,22E,26E)-2,6,10,14,19,23,27,31-octamethyldotriaconta-2,6,10,14,18,22,26,30-octaene |
| 2.5.1.148 | more |
squalene synthase enzyme diversification results in the production of specialized tetraterpenoid oils in race L of Botryococcus braunii |
| 2.5.1.148 | more |
the enzyme also acts as lycopaoctaene synthase and uses alternative C15 and C20 prenyl diphosphate substrates to produce combinatorial hybrid hydrocarbons, but almost exclusively uses GGPP in vivo. Squalene synthase enzyme diversification results in the production of specialized tetraterpenoid oils in race L of Botryococcus braunii. Race L produces the C40 tetraterpenoid hydrocarbon lycopadiene. Ozonolysis experiments suggest lycopatriene and lycopapentaene share an identical reduced C20 moiety with lycopadiene. NMR spectroscopy confirms identity and structure |
