EC Number   |
Natural Substrates |
|---|
   2.1.1.230 | S-adenosyl-L-methionine + adenosine1067 in 23S rRNA |
- |
| 2.1.1.230 | S-adenosyl-L-methionine + adenosine1067 in 23S rRNA |
Streptomyces azureus is the producer of the peptide antibiotic thiostrepton. Thiostrepton inhibits protein synthesis by binding to the complex of 23S rRNA and protein L-11 which blocks processes associated with the GTP-hydrolysis center of the ribosome including the binding of guanine nucleotides, elongation factor proteins and tRNA. 23S rRNA (adenosine1067-2'-O)-methyltransferase confers resistance to tthiostrepton |
| 2.1.1.230 | S-adenosyl-L-methionine + adenosine1067 in 23S rRNA |
the enzyme is involved in resistance to thiostrepton |
| 2.1.1.230 | S-adenosyl-L-methionine + adenosine1067 in 23S rRNA |
the methylase enzyme, responsible for autoimmunity in the thiostrepton producer Streptomyces azureus, renders ribosomes completely resistant to thiostrepton |
| 2.1.1.230 | S-adenosyl-L-methionine + adenosine1067 in 23S rRNA |
enzyme Tsr uses the co-substrate AdoMet to methylate the 23 S rRNA, presumably prior to the assembly of the 50 S subunit as the L11 and proposed Tsr binding surfaces are overlapping |
