EC Number   |
Natural Substrates |
|---|
   2.1.1.221 | more |
substrate specificity analysis in vivo, overview |
| 2.1.1.221 | more |
there are 19 unique tRNA species that contain a G at position 9 in yeast, and whose fully modified sequence is known, only 9 of these tRNA species are modified with m1G9 in wild-type cells. The in vitro methylation activity of yeast Trm10 is not sufficient to explain the observed pattern of modification in vivo, as additional tRNA species are substrates for Trm10 m1G9 methyltransferase activity, substrate specificity analysis in vivo, overview |
| 2.1.1.221 | S-adenosyl-L-methionine + guanine9 in tRNA |
- |
| 2.1.1.221 | S-adenosyl-L-methionine + guanine9 in tRNA |
the bifunctional enzyme catalyzes both methylation of guanine9 and methylation of adenine9 in tRNA |
| 2.1.1.221 | S-adenosyl-L-methionine + guanine9 in tRNA |
TRM10 is required for the catalysis of at least 9 of the 10 m1G modifications observed (tRNA(Trp), tRNA(Pro), tRNA(Val), tRNAi(Met), tRNA(Ile), tRNAICG(Arg), and both tRNAUCU(Arg) species) at position 9 in yeast. It is likely that Trm10p is also responsible for modification of G9 of tRNAAla |
| 2.1.1.221 | S-adenosyl-L-methionine + guanine9 in tRNAArg |
- |
| 2.1.1.221 | S-adenosyl-L-methionine + guanine9 in tRNAAsp |
- |
| 2.1.1.221 | S-adenosyl-L-methionine + guanine9 in tRNACysGCA |
- |
| 2.1.1.221 | S-adenosyl-L-methionine + guanine9 in tRNAGly |
Trm10 is specific for G9 of tRNAGly |
| 2.1.1.221 | S-adenosyl-L-methionine + guanine9 in tRNAGlyCCC |
- |
