EC Number |
Natural Substrates |
---|
1.14.14.51 | (-)-(S)-limonene + NADPH + O2 |
- |
1.14.14.51 | (-)-limonene + NADPH + O2 |
one of the key reactions of oxygenated monoterpenes, biosynthesis of (-)-carvone |
1.14.14.51 | (S)-limonene + NADPH + H+ + O2 |
convertion of (S)-limonene to cis- and trans-carveols in cells in 11% and 9% yields, respectively, after 6 h. The cells discriminate the (R) and (S) stereoisomers of the limonene and hydroxylate regioselectively at the 6-position of the (R) form |
1.14.14.51 | (S)-limonene + [reduced NADH-hemoprotein reductase] + O2 |
- |
1.14.14.51 | (S)-limonene + [reduced NADPH-hemoprotein reductase] + O2 |
- |
1.14.14.51 | more |
(S)-limonene is hydroxylated stereo- and regioselectively at its allylic position of the endocyclic C=C double bond by the cyanobacterial cells to its corresponding alcohol. The cells also show the ability for the enantio- and stereoselective cleavage of the epoxide group of (1S,2R,4R)-limonene oxide to give (1S,2S,4R)-limonene-1,2-diol. Biotransformation of (+)-limonene oxide produces (1S,2S,4R)-limonene-1,2-diol and (1S,4R)-limonene-1-ol-2-one in 32% and 16% yields, respectively, and (1R,2S,4R)-limonene oxide is recovered |