EC Number   |
Natural Substrates |
|---|
   1.14.14.29 | 25-hydroxycholesterol + NADPH + H+ + O2 |
- |
| 1.14.14.29 | 27-hydroxycholesterol + NADPH + H+ + O2 |
- |
| 1.14.14.29 | cholest-5-ene-3beta,25-diol + NADPH + H+ + O2 |
CYP7B1 oxysterol 7alpha-hydroxylase pathway synthesizes 25% to 30% of all bile acids |
| 1.14.14.29 | cholest-5-ene-3beta,25-diol + NADPH + H+ + O2 |
dietary cholesterol or colestipol do not affect oxysterol 7alpha-hydroxylase enzyme activity, mRNA, or protein levels |
| 1.14.14.29 | cholest-5-ene-3beta,25-diol + NADPH + H+ + O2 |
loss of the enzyme in liver is compensated for by increases in the synthesis of bile acids by other pathways |
| 1.14.14.29 | cholest-5-ene-3beta,27-diol + NADPH + H+ + O2 |
CYP7B1 oxysterol 7alpha-hydroxylase pathway synthesizes 25% to 30% of all bile acids |
| 1.14.14.29 | cholest-5-ene-3beta,27-diol + NADPH + H+ + O2 |
loss of the enzyme in liver is compensated for by increases in the synthesis of bile acids by other pathways |
| 1.14.14.29 | dehydroepiandrosterone + NADPH + H+ + o2 |
- |
| 1.14.14.29 | more |
both phorbol myristate acetate and cAMP decreases CYP7B1 activity by 60% and 34% respectively, in a time-dependent fashion. Changes in CYP7B1 messenger RNA levels correlate with changes in specific activities. CYP7B1 specific activity is highly regulated but does not seem to be rate limiting for bile acid synthesis |
| 1.14.14.29 | more |
regulation of CYP7B1 transcription by Sp1 may play a pivotal role in regulating oxysterol levels, which regulate cholesterol metabolism |
