EC Number |
Natural Substrates |
---|
1.14.14.20 | 2,4-dichlorophenol + FADH2 + O2 |
- |
1.14.14.20 | 2-chlorophenol + FADH2 + O2 |
poor substrate for isozyme PheA1(1) |
1.14.14.20 | 4-chlorophenol + FADH2 + O2 |
- |
1.14.14.20 | 4-methylphenol + FADH2 + O2 |
- |
1.14.14.20 | more |
enzyme components PheA2 and PheA1 show no protein-protein interaction |
1.14.14.20 | more |
substrate speccificities of the three isozymes, overview |
1.14.14.20 | phenol + FADH2 + O2 |
- |
1.14.14.20 | phenol + FADH2 + O2 |
a two-component enzyme system: the smaller flavin reductase PheA2 component catalyzes the NADH-dependent reduction of free FAD according to a ping pong bisubstrate-biproduct mechanism. The reduced FAD is then used by the larger oxygenase component PheA1 to hydroxylate phenols to the corresponding catechols |
1.14.14.20 | phenol + NADPH + O2 |
first step of phenol degradation |