EC Number |
Natural Substrates |
---|
1.1.1.422 | (+)-(1S,2S)-pseudoephedrine + NAD+ |
- |
1.1.1.422 | (1S,2S)-(+)-pseudoephedrine + NAD+ |
- |
1.1.1.422 | (S,R)-(+)-ephedrine + NAD+ |
- |
1.1.1.422 | (S,S)-(+)-pseudoephedrine + NAD+ |
- |
1.1.1.422 | 1-phenylpropan-1,2-dione + NAD+ |
- |
1.1.1.422 | 1-phenylpropan-1,2-dione + NAD+ |
PseDH overcomes the stability of the conjugated molecule PPD by reducing it to alpha-hydroxyketone (S)-PAC. Phenylacetylcarbinol (PAC) cannot be oxidized by these dehydrogenases and possibly undergoes a cleavage reaction to yield benzaldehyde and acetaldehyde |
1.1.1.422 | 1-phenylpropan-1,2-dione + NADH + H+ |
- |
1.1.1.422 | more |
PseDH is strictly stereospecific for the other diastereomers, (S,S)-(+)-pseudoephedrine and (S,R)-(+)-ephedrine. Conversion of all ephedrine isomers required the addition of NAD+ as an oxidant. Without a surplus oxidant or in the presence of NADP+, cell lysates do not significantly convert (pseudo)ephedrine |