EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Reference |
---|
4.2.1.32 | 22641 |
- |
2 * 22641 + 2 * 32589, DNA-derived values of TtdB and TtdA, quaternary structure of Escherichia coli K12 (W3110, wild-type) resembles that of the Pseudomonas putida enzyme |
5656 |
4.2.1.32 | 23000 |
- |
2 * 23000 + 2 * 27000, SDS-PAGE |
5654 |
4.2.1.32 | 27000 |
- |
2 * 23000 + 2 * 27000, SDS-PAGE |
5654 |
4.2.1.32 | 32589 |
- |
2 * 22641 + 2 * 32589, DNA-derived values of TtdB and TtdA, quaternary structure of Escherichia coli K12 (W3110, wild-type) resembles that of the Pseudomonas putida enzyme |
5656 |
4.2.1.32 | 39000 |
- |
sucrose density gradient centrifugation, inactive form. Aggregation of the four smaller units forms the active protein |
5653 |
4.2.1.32 | 100000 |
- |
gel filtration |
5654 |
4.2.1.32 | 105000 |
- |
gel filtration |
5656 |
4.2.1.32 | 110500 |
- |
DNA-derived values of TtdA and TtdB |
5656 |
4.2.1.32 | 145000 |
- |
sucrose density gradient centrifugation, active form. Enzyme exists as an aggregate of smaller protein or proteins, the latter possessing no enzyme activity |
5653 |