EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Reference |
---|
3.2.1.140 | 46000 |
- |
gel filtration |
26153 |
3.2.1.140 | 60000 |
- |
1 * 60000, SDS-PAGE |
26153 |
3.2.1.140 | 110000 |
- |
apparent molecular mass, SDS-PAGE |
695742 |
3.2.1.140 | 112000 |
- |
calculated mass |
695742 |
3.2.1.140 | 112000 |
- |
truncated enzyme without signal peptide and membrane anchor, amino acids 35-1064, SDS-PAGE, and calculated from amino acids |
695742 |
3.2.1.140 | 120000 |
- |
full enzyme, calculated from 350 bp PCR product of 1,112 amino acids |
695742 |
3.2.1.140 | 120000 |
- |
predicted mass, 1112 amino acid residues. The deduced amino acid sequence contains a signal peptide and a membrane anchor at the N-terminal and C-terminal ends. This result strongly suggests that LnbB is a membrane-tethered protein with a large extracellular region |
695742 |
3.2.1.140 | 161000 |
- |
2 * 161000, recombinant nontagged enzyme, SDS-PAGE |
732113 |
3.2.1.140 | 356000 |
- |
recombinant nontagged enzyme, gel filtration |
732113 |