EC Number   |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Reference |
|---|
    1.13.11.49 | 25000 |
- |
4 * 25000, SDS-PAGE |
285261 |
| 1.13.11.49 | 28400 |
- |
2 * 28400, recombinant mutant W155F, SDS-PAGE |
724812 |
| 1.13.11.49 | 28400 |
- |
4 * 28400, SDS-PAGE |
685244 |
| 1.13.11.49 | 30000 |
- |
4 * 30000 Da, elution position on a gel-filtration column using Stokes radius to determine molecular weight points to globular tetramer, collisional activation of the ions ejects one monomer of the pentamer, so that low-charged tetramer counter complex ions are also observed with native mass spectrometry |
699577 |
| 1.13.11.49 | 30000 |
- |
5 * 30000 Da, tandem mass spectrometry, native mass spectrometry, active state in solution |
699577 |
| 1.13.11.49 | 30000 |
- |
6 * 30000, multiple anomalous dispersion crystal structure, one heme per monomer (histidine 170 axial heme ligand), arginine 183 probably with essential role in substrate positioning and activation, activity of the hexamer not measurable because of the presence of the thiocyanate inhibitor, each monomer appears to be acting on its own, therefore, different quaternary states may be biologically relevant depending on protein concentration, pH, or other factors |
699577 |
| 1.13.11.49 | 30420 |
- |
monomer without heme, native mass spectrometry, enzyme buffer-exchanged to 50 mM ammonium acetate, pH 6.8, with centrifugal filter units with 30 kDa cutoff |
699577 |
| 1.13.11.49 | 31000 |
- |
4 * 31000, heme content 1.5 mol Fe2+/mol enzyme (calculated from Soret peak shift measurement), 2.2 mol Fe2+/mol enzyme (metal analysis by ICP-MS) |
697991 |
| 1.13.11.49 | 31030 |
- |
monomer with heme, native mass spectrometry, enzyme buffer-exchanged to 50 mM ammonium acetate, pH 6.8, with centrifugal filter units with 30 kDa cutoff |
699577 |
| 1.13.11.49 | 31032 |
- |
1 * 30420 without heme, 1 * 31032 with heme |
699577 |
