EC Number   |
Metals/Ions |
Reference |
|---|
   4.2.3.46 | K+ |
30-50 mM, enhances activity 5fold. Km: 3 mM. Addition of K+ reduces monoterpene synthase activity |
700662 |
| 4.2.3.46 | K+ |
500 mM are included in monoterpene synthase assay |
694765 |
| 4.2.3.46 | K+ |
activity is dependent on K+, the potassium binding region is defined |
693257 |
| 4.2.3.46 | K+ |
in presence of 50 mM potassium, the enzyme activity increases 15fold as compared to the activity in an assay devoid of potassium |
716469 |
| 4.2.3.46 | K+ |
MdAFS1 retains up to 12% of its activity in the absence of K+, enzyme contains K+ binding region, MdAFS1 exhibits a type II K+ response, MdAFS1 is not absolutely dependent upon M+ its unequivocal classification as type I or type II K+ activated, or that of any other terpene synthases, will not be possibl, then type I enzymes can exhibit type II kinetics and vice versa. |
693257 |
| 4.2.3.46 | K+ |
required |
748904 |
| 4.2.3.46 | Mg2+ |
10 mM are included in farnesyl diphosphate activity assay |
693257 |
| 4.2.3.46 | Mg2+ |
10 mM are included in sesquiterpene synthase assay |
694765 |
| 4.2.3.46 | Mg2+ |
Km: 0.248 mM, divalent cation required, preference for Mg2+. Maximal velocities with Mn2+ is about 50% of that with Mg2+ |
699267 |
| 4.2.3.46 | Mg2+ |
Km: 0.7 mM |
700662 |
