EC Number |
Metals/Ions |
Reference |
---|
4.1.99.2 | K+ |
5-10 mM, accelerates activity |
33465 |
4.1.99.2 | K+ |
activating |
678401 |
4.1.99.2 | K+ |
activation |
651062 |
4.1.99.2 | K+ |
catalytically important |
678198 |
4.1.99.2 | K+ |
K+ or NH4+ required for maximal activity |
33459 |
4.1.99.2 | more |
divalent cations (Mg2+, Ca2+, Ba2+, and Sr2+) do not activate TPL |
678198 |
4.1.99.2 | more |
the enzyme requires a monovalent cation, either K+, NH4+, Rb+ or Cs+ for activity, with Na+ and Li+ giving little or no activity. The cation is bound by the gamma-carboxylate of a conserved glutamate residue Glu69 of the TPL peptide backbone carbonyl oxygens, and waters, and is located about 10 A from cofactor pyridoxal 5'-phosphate |
747245 |
4.1.99.2 | NH4+ |
5-10 mM, accelerates activity |
33465 |
4.1.99.2 | NH4+ |
K+ or NH4+ required for maximal activity |
33459 |