EC Number   |
Metals/Ions |
Reference |
|---|
   3.1.21.2 | Ca2+ |
stimulates activity |
730742 |
| 3.1.21.2 | Ca2+ |
the enzyme requires the presence of Mg2+ and, to lesser extent, Ca2+ and Mn2+ for the DNA repair activity |
750388 |
| 3.1.21.2 | CaCl2 |
8% of the activity found compared to optimal Mg2+ concentration |
134240 |
| 3.1.21.2 | Co2+ |
required for activity |
716663 |
| 3.1.21.2 | Co2+ |
the enzyme exhibits a non-linear dependence in the presence of Co2+. The AP site cleavage activity increases from 0.1 to 0.5 mM and then rapidly decreases from 0.5 to 10 mM CoCl2 |
750388 |
| 3.1.21.2 | CoCl2 |
can substitute for MgCl2, optimal concentration: 0.01 M, activity 27% higher than at optimal MgCl2 concentration |
134240 |
| 3.1.21.2 | KCl |
the enzyme requires a low ionic strength |
750388 |
| 3.1.21.2 | KCl |
the enzyme requires high ionic strength, 200 mM KCl |
750388 |
| 3.1.21.2 | Mg2+ |
- |
683117 |
| 3.1.21.2 | Mg2+ |
absolutely required for nicking activity |
666387 |
