EC Number |
Metals/Ions |
Reference |
---|
3.1.12.1 | iron-sulfur centre |
contains a [4Fe-4S] cluster |
726307 |
3.1.12.1 | iron-sulfur centre |
near the active site, each enzyme protomer contains one [4Fe-4S] cluster, which is important for both maintaining the decameric state and nuclease activity |
725211 |
3.1.12.1 | K+ |
nuclease activity of the enzyme is maximal in the presence of 20-200 mM KCl |
725211 |
3.1.12.1 | Mg2+ |
cleavage of a 15T DNA oligonucleotide by Sso0001 is observed in the presence of either magnesium or manganese, yielding a cluster of products of around 15 nt in size. A 20U RNA oligonucleotide is cleaved in the presence of manganese but not magnesium |
726307 |
3.1.12.1 | Mg2+ |
nuclease activity of the enzyme is maximal in the presence of Mg2+ or Mn2+ (5 mM) |
725211 |
3.1.12.1 | Mn2+ |
cleavage of a 15T DNA oligonucleotide by Sso0001 is observed in the presence of either magnesium or manganese, yielding a cluster of products of around 15 nt in size. A 20U RNA oligonucleotide is cleaved in the presence of manganese but not magnesium |
726307 |
3.1.12.1 | Mn2+ |
nuclease activity of the enzyme is maximal in the presence of Mg2+ or Mn2+ (5 mM). Mn2+ is bound in the active site located inside the internal tunnel |
725211 |
3.1.12.1 | more |
Ca2+ does not support the nuclease activity |
725211 |