EC Number   |
Metals/Ions |
Reference |
|---|
   2.7.7.50 | Ca2+ |
can partially replace Mg2+ in activation |
643480 |
| 2.7.7.50 | Co2+ |
30% of the activity with Mg2+ |
695159 |
| 2.7.7.50 | K+ |
stimulates, maximal activation at 6 mM KCl |
643480 |
| 2.7.7.50 | Mg2+ |
- |
209891, 643470, 690946 |
| 2.7.7.50 | Mg2+ |
10 mM used in assay conditions |
761967 |
| 2.7.7.50 | Mg2+ |
25% of the activity with Mn2+ |
643466 |
| 2.7.7.50 | Mg2+ |
both reaction steps are dependent on divalent metal ions |
695159 |
| 2.7.7.50 | Mg2+ |
can partially replace Mn2+ in activation |
643466, 643475, 643476 |
| 2.7.7.50 | Mg2+ |
enzyme-GMP intermediate formation is strictly dependent on divalent cations, satisfied by Mn2+ or Mg2+, Mn2+ is slightly preferred |
643486, 643490 |
| 2.7.7.50 | Mg2+ |
magnesium binding likely activates the lysine nucleophile by increasing its acidity and by biasing the deprotonated nucleophile into conformations conducive to intermediate formation |
721658 |
