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EC Number	Metals/Ions	Commentary	Reference
2.5.1.86	Ca2+	absolute requirement for divalent cations. Enzyme activity is optimal in the presence of 1 mM Mg2+. MnCl2 at 0.1 mM also supports the activity. CaCl2 and ZnCl2 are much less effective	704266
2.5.1.86	Mg2+	2 mM used in assay conditions	758676
2.5.1.86	Mg2+	absolute requirement for divalent cations. Enzyme activity is optimal in the presence of 1 mM Mg2+. MnCl2 at 0.1 mM also supports the activity. CaCl2 and ZnCl2 are much less effective	704266
2.5.1.86	Mg2+	contains Mg2+	758604
2.5.1.86	Mg2+	required	729897, 737596
2.5.1.86	Mn2+	absolute requirement for divalent cations. Enzyme activity is optimal in the presence of 1 mM Mg2+. MnCl2 at 0.1 mM also supports the activity. CaCl2 and ZnCl2 are much less effective	704266
2.5.1.86	Zn2+	absolute requirement for divalent cations. Enzyme activity is optimal in the presence of 1 mM Mg2+. MnCl2 at 0.1 mM also supports the activity. CaCl2 and ZnCl2 are much less effective	704266

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Release 2023.1 (January 2023)