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EC Number	Metals/Ions	Commentary	Reference
2.4.1.22	Ca2+	25% of the activation with Mn2+	488939
2.4.1.22	Mg2+	25% of the activation with Mn2+	488939
2.4.1.22	Mn2+	-	488939, 488942
2.4.1.22	Mn2+	not replaceable by Mg2+, Ca2+	488942
2.4.1.22	Mn2+	required	659529
2.4.1.22	Mn2+	required, Km: 0.2 mM	488944
2.4.1.22	Mn2+	required, optimal metal cofactor at 0.1 mM MnCl2	657536
2.4.1.22	Mn2+	required, partially replaceable by Mg2+, Ca2+	488939

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Release 2023.1 (January 2023)