EC Number   |
Metals/Ions |
Reference |
|---|
   2.4.1.212 | Co2+ |
18% and 39% of the wild-type GlcUA-transferase and GlcNAc-transferase activity with Mn2+, respectively, 0.2 mM |
658841 |
| 2.4.1.212 | Co2+ |
2% as effective as Mn2+ at similar concentrations |
395250 |
| 2.4.1.212 | K+ |
coactivation occurs if K+ is applied together with Mn2+ or Mg2+. With Mn2+, a high UDP reaction rate and high molecular mass of hyaluronic acid (2.45 MDa after 8 h) are achieved. With Mg2+, a low reaction rate and low molecular mass (1.55 MDa after 8 h) are reached. If 10 mM K+ are added to 15 mM Mg2+, a significant increase of the reaction rate by a factor of 2.7 is observed, and the molecular mass is doubled (3.11 MDa after 8 h). The coactivating effect of K+ together with Mn2+ is less pronounced |
756468 |
| 2.4.1.212 | KCl |
activates at 50 mM, slightly more effective than NaCl |
658093 |
| 2.4.1.212 | Mg2+ |
- |
395252, 659346, 659455 |
| 2.4.1.212 | Mg2+ |
20% as effective as Mn2+ at similar concentrations |
395250 |
| 2.4.1.212 | Mg2+ |
66% and 77% of the wild-type GlcUA-transferase and GlcNAc-transferase activity with Mn2+, respectively, 0.2 mM |
658841 |
| 2.4.1.212 | Mg2+ |
a nucleotide with 2 phosphate groups and complexed with a Mg2+ ion is absolutely required for activity |
659175 |
| 2.4.1.212 | Mg2+ |
Mg2+ activates with an optimum of 15-20 mM leading to about half of the yield obtained with Mn2+ after 8 h |
756468 |
| 2.4.1.212 | Mg2+ |
required |
658093, 658946, 722359, 722741, 722782, 722816, 736643 |
