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EC Number	Metals/Ions	Commentary	Reference
2.2.1.3	Mg2+	5 mM, essential for effective binding of thiamine diphosphate to enzyme molecule	486050
2.2.1.3	Mg2+	activation	486048
2.2.1.3	Mg2+	requirement	486047, 486051, 486057
2.2.1.3	Mn2+	activation, replaces Mg2+ to some extent	486051
2.2.1.3	more	-	486048
2.2.1.3	more	no activation by Ca2+	486048, 486050
2.2.1.3	more	no metal cations	486048

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Release 2023.1 (January 2023)