EC Number   |
Metals/Ions |
Reference |
|---|
    2.1.1.104 | Ca2+ |
contains Ca2+ |
758038 |
| 2.1.1.104 | Ca2+ |
in the active site of the enzyme surrounded by an octahedral arrangement of hydroxyl and carboxyl ligands, mediates the deprotonation of the caffeoyl 3-hydroxyl group and maintains the resultant oxoanion in close proximity to the reactive methyl group of S-adenosyl-L-methionine allowing for facile transmethylation to occur, restores activity of EDTA treated CCoAOMT to 100% the level of untreated CCoAOMT |
670583 |
| 2.1.1.104 | Co2+ |
0.15 mM, about 2fold increase of the rate of methylation of caffeoyl-CoA. Replacing Co2+ for Mg2+ drastically shifts the substrate preferences towards caffeic, 5-hydroxyferulic and 3,4-dihydroxybenzoic acid, which are not at all accepted in assays with Mg2+ as cofactor |
668599 |
| 2.1.1.104 | Co2+ |
Co2+ results in recovery of 82% of OMT-15 and 41% of OMT-17 activity compared to the addition of Mg2+ |
700855 |
| 2.1.1.104 | Mg2+ |
- |
485078 |
| 2.1.1.104 | Mg2+ |
5 mM used in assay conditions |
758057 |
| 2.1.1.104 | Mg2+ |
activation, 0.2 mM |
485080 |
| 2.1.1.104 | Mg2+ |
dependent on |
700855 |
| 2.1.1.104 | Mg2+ |
required |
668599 |
| 2.1.1.104 | Mg2+ |
required for activity |
757935 |
