Fe2+
aerobic purification results in inactive protein, which can only partly be reactivated with Fe2+ and thiol
Fe2+
Km for ferrous ammonium sulfate: 0.004 mM
Fe2+
maximal activity at 0.2-0.9 mM
Fe2+
storage of the protein for 6 months leads to almost complete loss of its activity, which can be fully restored by the reactivation with Fe2+ and thiol