EC Number   |
Metals/Ions |
Reference  |
|---|
   3.4.21.62 | more |
enzyme contains no metal ion |
29425 |
| 3.4.21.62 | Ca2+ |
as found in other Bacillus subtilisins, the structure of wild-type Savinase contains 2 calcium ion-binding sites |
29435 |
| 3.4.21.62 | Ca2+ |
bound calcium ions play a key role in protecting against autolysis and thermal denaturation |
649692, 652805 |
| 3.4.21.62 | Cd2+ |
stabilizes |
649692 |
| 3.4.21.62 | Sr2+ |
increases thermostability, but to a significantly lower degree than Ca2+ |
649692 |
| 3.4.21.62 | more |
shows no evidence of Ca2+ activation |
649830 |
| 3.4.21.62 | Ca2+ |
stabilizing mutations are usually calcium-dependent in their stabilizing effect |
650018 |
| 3.4.21.62 | more |
strain DJ-4, not significantly affected by Ca2+, Co2+, Mg2+, or Mn2+ |
650609 |
| 3.4.21.62 | Ca2+ |
increase on activity by 20% |
667756 |
| 3.4.21.62 | Cu2+ |
5 mM, 40% increase in activity |
669738 |
