EC Number   |
Metals/Ions |
Reference |
|---|
   1.13.11.66 | copper |
copper content is 0.12 atoms per monomer |
745802 |
| 1.13.11.66 | Fe2+ |
a Fe2+-dependent dioxygenase, selectively utilizes Fe2+for its catalytic reaction. Four residues of enzyme PnpD, His256, Asn258, Glu262, and His303, coordinate the iron ion |
745331 |
| 1.13.11.66 | Fe2+ |
required for catalysis, each of the four beta subunits in the asymmetric unit of the enzyme crystal binds one Fe(II) ion. The iron ion in each beta subunit is coordinated to three protein residues, His258, Glu264, and His305 and a water molecule |
744430 |
| 1.13.11.66 | Fe3+ |
binding structure, overview |
745331 |
| 1.13.11.66 | Iron |
1.4 mol of Fe2+ per mol of enzyme |
718555 |
| 1.13.11.66 | Iron |
10fold activation by Fe2+, each subunit binds one molecule of 4-hydroxybenzoate and one molecule of iron |
719714 |
| 1.13.11.66 | Iron |
Fe2+ is absolutely required, Km value 0.0046 mM |
718727 |
| 1.13.11.66 | Iron |
Fe2+ is required |
718831 |
| 1.13.11.66 | Iron |
iron content is 0.96 atoms per monomer |
745802 |
| 1.13.11.66 | Iron |
preincubation in presence of 0.1 mM Fe2+ and 1 mM Mn2+ increases activity by 10fold |
719327 |
