EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    6.4.1.1 | -999 |
- |
more |
0.5 mM Mg2+: Vmax (micromol/mg/min) 1.16, Ka (acetyl-CoA) 0.038 mM/1 mM Mg2+: Vmax (micromol/mg/min) 1.39, Ka (acetyl-CoA) 0.026 mM/1.5 mM Mg2+: Vmax (micromol/mg/min) 2.59, Ka (acetyl-CoA) 0.025 mM/3 mM Mg2+: Vmax (micromol/mg/min) 5.7, Ka (acetyl-CoA) 0.01 mM |
726972 |
| 6.4.1.1 | -999 |
- |
more |
stopped flow kinetics using fluorescent ATP analogue formycin A-5'-triphosphate, presence of biotin enhanced binding, kinetics of free biotin carboxylation |
692637 |
| 6.4.1.1 | 0.0028 |
- |
ADP |
wild-type, MgADP phosphorylation, pH 7.8, 30°C |
727040 |
| 6.4.1.1 | 0.008 |
- |
ADP |
mutant H216N, MgADP phosphorylation, pH 7.8, 30°C |
727040 |
| 6.4.1.1 | 0.009 |
- |
ATP |
wild-type, MgATP cleavage, pH 7.8, 30°C |
727040 |
| 6.4.1.1 | 0.018 |
- |
ATP |
pH 7.5, 25°C, wild-type, 10 mM biotin, pyruvate carboxylation |
726972 |
| 6.4.1.1 | 0.022 |
- |
ATP |
pH 7.5, 25°C, wild-type, no biotin, pyruvate carboxylation |
726972 |
| 6.4.1.1 | 0.027 |
- |
ATP |
with 10 mM free Mg2+ |
1795 |
| 6.4.1.1 | 0.039 |
- |
pyruvate |
- |
663285 |
| 6.4.1.1 | 0.04 |
- |
ATP |
- |
1760 |
