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Results 1 - 10 of 41 > >>
EC Number KM Value [mM] KM Value Maximum [mM] Substrate Commentary Reference
Show all pathways known for 6.1.1.5Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.5-999 - more kinetic analysis, rapid equilibrium determinations, steady-state kinetics. The analysis strongly suggests an additional activation step, formation of a new isoleucyl-AMP before the isoleucyl-tRNA is freed from the enzyme. The removal of Ile-tRNA is possible without the formation of Ile-AMP if both isoleucine and ATP are bound to the E-Ile-tRNA complex, but this route covers only 11% of the total formation of Ile-tRNA. In addition to the Mg2+ in MgATP or Mg-diphosphate, only two tRNA-bound Mg2+ are required to explain the magnesium dependence in the best-fit mechanism. The first Mg2+ might be present in all steps before the second activation and is obligatory in the first reorganizing step and transfer step. The second Mg2+ is present only at the transfer step, whereas elsewhere it prevents the reaction, including the activation reaction 744920
Show all pathways known for 6.1.1.5Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.5-999 - more Km value of mutant enzynes with altered metal-binding sites 452
Show all pathways known for 6.1.1.5Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.5-999 - more Km value of structural analogs of adenosine 5'-triphosphate in the aminoacylation reaction 469
Show all pathways known for 6.1.1.5Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.5-999 - more reaction kinetics 651903
Show all pathways known for 6.1.1.5Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.5-999 - more single-turnover kinetic analysis 745362
Show all pathways known for 6.1.1.5Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.5-999 - more single-turnover kinetic analysis, activation kinetics of isoleucine and valine by ScIleRS at 30°C 745362
Show all pathways known for 6.1.1.5Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.5-999 - more single-turnover kinetic analysis, activation kinetics of isoleucine and valine by SgIleRS at 30°C 745362
Show all pathways known for 6.1.1.5Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.5-999 - more steady-state kinetics of activation of cognate L-Ile and noncognate amino acid L-Val by Y59 IleRS mutant variants, and transfer kinetics, as well as tRNA-dependent hydrolysis of cognate isoleucyl-AMP, overview 744324
Show all pathways known for 6.1.1.5Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.5-999 - more steady-state parameters for tRNA-independent pre-transfer editing by IleRS and its mutants determined by varying concentrations of noncognate valine, overview. Kinetic method to distinguish among three models for pre-transfer editing by IleRS, overview 715519
Show all pathways known for 6.1.1.5Display the word mapDisplay the reaction diagram Show all sequences 6.1.1.50.00000004 - L-isoleucine pH and temperature not specified in the publication 727946
Results 1 - 10 of 41 > >>