EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
6.1.1.5 | -999 |
- |
more |
kinetic analysis, rapid equilibrium determinations, steady-state kinetics. The analysis strongly suggests an additional activation step, formation of a new isoleucyl-AMP before the isoleucyl-tRNA is freed from the enzyme. The removal of Ile-tRNA is possible without the formation of Ile-AMP if both isoleucine and ATP are bound to the E-Ile-tRNA complex, but this route covers only 11% of the total formation of Ile-tRNA. In addition to the Mg2+ in MgATP or Mg-diphosphate, only two tRNA-bound Mg2+ are required to explain the magnesium dependence in the best-fit mechanism. The first Mg2+ might be present in all steps before the second activation and is obligatory in the first reorganizing step and transfer step. The second Mg2+ is present only at the transfer step, whereas elsewhere it prevents the reaction, including the activation reaction |
744920 |
6.1.1.5 | -999 |
- |
more |
Km value of mutant enzynes with altered metal-binding sites |
452 |
6.1.1.5 | -999 |
- |
more |
Km value of structural analogs of adenosine 5'-triphosphate in the aminoacylation reaction |
469 |
6.1.1.5 | -999 |
- |
more |
reaction kinetics |
651903 |
6.1.1.5 | -999 |
- |
more |
single-turnover kinetic analysis |
745362 |
6.1.1.5 | -999 |
- |
more |
single-turnover kinetic analysis, activation kinetics of isoleucine and valine by ScIleRS at 30°C |
745362 |
6.1.1.5 | -999 |
- |
more |
single-turnover kinetic analysis, activation kinetics of isoleucine and valine by SgIleRS at 30°C |
745362 |
6.1.1.5 | -999 |
- |
more |
steady-state kinetics of activation of cognate L-Ile and noncognate amino acid L-Val by Y59 IleRS mutant variants, and transfer kinetics, as well as tRNA-dependent hydrolysis of cognate isoleucyl-AMP, overview |
744324 |
6.1.1.5 | -999 |
- |
more |
steady-state parameters for tRNA-independent pre-transfer editing by IleRS and its mutants determined by varying concentrations of noncognate valine, overview. Kinetic method to distinguish among three models for pre-transfer editing by IleRS, overview |
715519 |
6.1.1.5 | 0.00000004 |
- |
L-isoleucine |
pH and temperature not specified in the publication |
727946 |