EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
5.4.99.14 | 0.028 |
- |
1-methyl-3,7-dioxo-2,6-dioxabicyclo[3.3.0]octane |
- |
3483 |
5.4.99.14 | 0.045 |
- |
(4S)-4-methylmuconolactone |
mutant enzyme Y39F, in phosphate buffer, 50 mM, pH 7.3 |
704578 |
5.4.99.14 | 0.052 |
- |
(4S)-4-methylmuconolactone |
wild type enzyme, in phosphate buffer, 50 mM, pH 7.3 |
704578 |
5.4.99.14 | 0.0972 |
- |
(4S)-4-methylmuconolactone |
mutant enzyme C67S, in phosphate buffer, 50 mM, pH 7.3 |
704578 |
5.4.99.14 | 0.17 |
- |
1-methyl-bislactone |
- |
3482 |
5.4.99.14 | 0.176 |
- |
4-carboxymethyl-4-methylbut-2-en-1,4-olide |
- |
3483 |
5.4.99.14 | 0.23 |
- |
4-carboxymethyl-4-methylbut-2-en-1,4-olide |
- |
3482 |
5.4.99.14 | 0.4583 |
- |
(4S)-4-methylmuconolactone |
mutant enzyme H52A, in phosphate buffer, 50 mM, pH 7.3 |
704578 |