EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
5.4.99.13 | -999 |
- |
more |
the pivalyl-CoA mutase activity of engineered PCM shows a sigmoidal dependence on the concentration of isolvaleryl-CoA |
748193 |
5.4.99.13 | 0.0201 |
- |
isobutyryl-CoA |
recombinant enzyme, at 37°C |
704700 |
5.4.99.13 | 0.0453 |
- |
isobutyryl-CoA |
recombinant enzyme, in the presence of GDP, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C |
704700 |
5.4.99.13 | 0.0508 |
- |
isobutyryl-CoA |
recombinant enzyme, in the presence of GTP, in 50 mM sodium phosphate buffer, pH 7.5, 250 mM NaCl at 37°C |
704700 |
5.4.99.13 | 0.054 |
- |
2-methylpropanoyl-CoA |
pH 7.4, 30ºC |
648802 |
5.4.99.13 | 0.057 |
- |
butanoyl-CoA |
pH 7.4, 30ºC |
648802 |
5.4.99.13 | 0.2 |
- |
isovaleryl-CoA |
recombinant engineered PCM-F mutant, pH 7.5, 37°C |
748193 |
5.4.99.13 | 0.36 |
- |
2-methylpropanoyl-CoA |
recombinant mutant F598I, pH 7.5, 37°C |
748193 |
5.4.99.13 | 0.37 |
- |
isovaleryl-CoA |
recombinant engineered PCM mutant, pH 7.5, 37°C |
748193 |
5.4.99.13 | 0.45 |
- |
2-methylpropanoyl-CoA |
recombinant mutant F598A, pH 7.5, 37°C |
748193 |