EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
4.1.3.39 | 0.0044 |
- |
(S)-4-hydroxy-2-oxopentanoate |
in 100 mM HEPES (pH 8.0), 0.4 mM NADH, 1 mM MgCl2, at 25°C |
724380 |
4.1.3.39 | 0.0048 |
- |
(S)-4-hydroxy-2-oxohexanoate |
in 100 mM HEPES (pH 8.0), 0.4 mM NADH, 1 mM MgCl2, at 25°C |
724380 |
4.1.3.39 | 0.012 |
- |
(4R)-4-hydroxy-2-oxopentanoate |
Y290S mutant protein, pH 8.0, 25°C |
714244 |
4.1.3.39 | 0.013 |
- |
(4R)-4-hydroxy-2-oxopentanoate |
Y290F mutant protein, pH 8.0, 25°C |
714244 |
4.1.3.39 | 0.013 |
- |
(4S)-4-hydroxy-2-oxopentanoate |
Y290F mutant protein, pH 8.0, 25°C |
714244 |
4.1.3.39 | 0.013 |
- |
(4S)-4-hydroxy-2-oxopentanoate |
Y290S mutant protein, pH 8.0, 25°C |
714244 |
4.1.3.39 | 0.089 |
- |
(4S)-4-hydroxy-2-oxopentanoate |
wild type protein, pH 8.0, 25°C |
714244 |
4.1.3.39 | 0.089 |
- |
(S)-4-hydroxy-2-oxopentanoate |
in the presence of NADH, in 100 mM HEPES pH 8.0, at 25°C |
724341 |
4.1.3.39 | 0.158 |
- |
(S)-4-hydroxy-2-oxopentanoate |
in the absence of NADH, in 100 mM HEPES pH 8.0, at 25°C |
724341 |
4.1.3.39 | 0.222 |
- |
(4S)-4-hydroxy-2-oxopentanoate |
H20A mutant protein, pH 8.0, 25°C |
714244 |