EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   4.1.2.48 | 0.000002 |
- |
L-phenylserine |
recombinant wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0) |
681356 |
| 4.1.2.48 | 0.0000026 |
- |
L-phenylserine |
mutant enzyme H177Y, in 50 mM Tris-HCl buffer (pH 8.0) |
681356 |
| 4.1.2.48 | 0.00016 |
- |
L-Thr |
mutant enzyme H177Y, in 50 mM Tris-HCl buffer (pH 8.0) |
681356 |
| 4.1.2.48 | 0.00018 |
- |
L-Thr |
recombinant wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0) |
681356 |
| 4.1.2.48 | 0.0002 |
- |
L-threo-3,4-dihydroxyphenylserine |
recombinant wild type enzyme, in 50 mM Tris-HCl buffer (pH 8.0) |
681356 |
| 4.1.2.48 | 0.00023 |
- |
L-threo-3,4-dihydroxyphenylserine |
mutant enzyme H177Y, in 50 mM Tris-HCl buffer (pH 8.0) |
681356 |
| 4.1.2.48 | 0.027 |
- |
L-4-hydroxythreonine |
pH 8.0, 25°C |
716291 |
| 4.1.2.48 | 0.052 |
- |
L-allo-threonine |
pH 8.0, 25°C |
716291 |
| 4.1.2.48 | 0.12 |
- |
DL-threo-phenylserine |
pH 8.0, 30°C |
5206 |
| 4.1.2.48 | 0.2 |
- |
L-allo-threonine |
mutant H126NF, pH 7.0, 30°C |
727485 |
