EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   4.1.2.47 | -999 |
- |
more |
- |
651012 |
| 4.1.2.47 | -999 |
- |
more |
a kinetic investigation based on the rate curve method |
662488 |
| 4.1.2.47 | -999 |
- |
more |
wild-type enzyme shows very low activity with 4-hydroxymandelonitrile, activity of mutant enzyme W128A is increased 450fold compared to wild-type value (Km-value for mutant enzyme W128A is 0.625 mM) |
653798 |
| 4.1.2.47 | 0.2 |
- |
1-naphthalene carboxaldehyde |
pH 4.2, 22°C |
747168 |
| 4.1.2.47 | 0.28 |
- |
(R)-mandelonitrile |
pH 4.23, 25°C, mutant enzyme H103C |
747534 |
| 4.1.2.47 | 0.34 |
- |
(S)-mandelonitrile |
pH 4.23, 25°C, mutant enzyme H103C |
747534 |
| 4.1.2.47 | 0.4 |
- |
2-nitro-1-phenylethanol |
mutant enzyme F125T/L146M, pH and temperature not specified in the publication |
747531 |
| 4.1.2.47 | 0.4 |
- |
rac-2-nitro-1-phenylethanol |
pH 5.0, temperature not specified in the publication, mutant enzyme F125T/L146M |
747531 |
| 4.1.2.47 | 0.47 |
- |
(S)-mandelonitrile |
pH 4.23, 25°C, mutant enzyme H103C/N156D |
747534 |
| 4.1.2.47 | 0.49 |
- |
(S)-mandelonitrile |
pH 4.23, 25°C, mutant enzyme H103C/N156G |
747534 |
