EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    4.1.1.72 | 0.00063 |
- |
thiamine diphosphate |
wild-type, pH 7.5, 30°C, overall reaction |
656230 |
| 4.1.1.72 | 0.001 |
- |
L-3-Methyl-2-oxopentanoate |
and 2-oxoisopentanoate, 2-oxoisohexanoate, value below, 30°C, pH 7.5 |
648370 |
| 4.1.1.72 | 0.0066 |
- |
thiamine diphosphate |
wild-type, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction |
656230 |
| 4.1.1.72 | 0.015 |
- |
thiamine diphosphate |
mutant D295A, pH 7.5, 30°C, overall reaction |
656230 |
| 4.1.1.72 | 0.016 |
- |
thiamine diphosphate |
mutant D295A, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction |
656230 |
| 4.1.1.72 | 0.018 |
- |
thiamine diphosphate |
mutant Y300F, pH 7.5, 30°C, overall reaction |
656230 |
| 4.1.1.72 | 0.026 |
- |
thiamine diphosphate |
mutant R301A, pH 7.5, 30°C, overall reaction |
656230 |
| 4.1.1.72 | 0.026 |
- |
thiamine diphosphate |
mutant Y300A, pH 7.5, 30°C, overall reaction |
656230 |
| 4.1.1.72 | 0.027 |
- |
3-methyl-2-oxobutanoate |
pH 7.0, 55°C |
747761 |
| 4.1.1.72 | 0.028 |
- |
thiamine diphosphate |
mutant Y300F, pH 7.5, 30°C, E1b-catalyzed decarboxylation reaction |
656230 |
