EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    4.1.1.43 | 0.0051 |
- |
phenylpyruvate |
- |
4562, 4563 |
| 4.1.1.43 | 0.03 |
- |
Indole-3-pyruvate |
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C |
714975 |
| 4.1.1.43 | 0.054 |
- |
phenylpyruvate |
mutant enzyme Q448W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C |
714975 |
| 4.1.1.43 | 0.062 |
- |
phenylpyruvate |
strain CEN.PK 608-4B |
649388 |
| 4.1.1.43 | 0.09 |
- |
2-oxo-4-phenylbutanoic acid |
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C |
714975 |
| 4.1.1.43 | 0.09 |
- |
4-hydroxyphenylpyruvate |
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C |
714975 |
| 4.1.1.43 | 0.1 |
- |
phenylpyruvate |
strain CEN.PK 113-7D |
649388 |
| 4.1.1.43 | 0.1 |
- |
phenylpyruvate |
wild type enzyme, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C |
714975 |
| 4.1.1.43 | 0.13 |
- |
Indole-3-pyruvate |
in 10 mM MES, 2.5 mM MgSO4, 0.1 mM thiamine diphosphate, 0.35 mM NADH, and 0.125 units alcohol dehydrogenase, at pH 6.5 and 30°C |
680508 |
| 4.1.1.43 | 0.17 |
- |
2-oxohexanoic acid |
mutant enzyme M624W, in 100 mM KPO4 buffer (pH 7.0), 1 mM MgSO4, 0.5 mM thiamine diphosphate, at pH 7.0 and 30°C |
714975 |
