EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
3.8.1.8 | -999 |
- |
more |
kinetic parameters of diverse mutants relative to the wild-type values, overview |
733315 |
3.8.1.8 | -999 |
- |
more |
reduction in Km observed in the AtzA variants has allowed the full kinetic profile for the AtzA-catalyzed dechlorination of atrazine to be determined for the first time, revealing the hitherto-unreported substrate cooperativity in AtzA |
690560 |
3.8.1.8 | 0.02 |
- |
2-(N-ethylamino)-4-hydroxy-6-(N-isopropylamino)-1,3,5-triazine |
deamination |
687391 |
3.8.1.8 | 0.02 |
- |
hydroxyatrazine |
- |
687391 |
3.8.1.8 | 0.0238 |
- |
Atrazine |
- |
687101 |
3.8.1.8 | 0.029 |
- |
2,4-diisopropylamino-6-hydroxy-1,3,5-triazine |
deamination |
687391 |
3.8.1.8 | 0.04 |
- |
6-hydroxy-4-(N-isopropylamino)-2-(N-methylamino)-1,3,5-triazine |
deamination |
687391 |
3.8.1.8 | 0.049 |
- |
Atrazine |
A216A/T217D/T219E/A220A/D250D, site-directed mutagenesis, AtzA-variant 288, kinetic data obtained using 4.6 to 153 microM atrazine and 10 nM enzyme |
690560 |
3.8.1.8 | 0.049 |
- |
Atrazine |
T217D/T219E, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm |
690560 |
3.8.1.8 | 0.062 |
- |
Atrazine |
A216G/T217D/T219G/A220H/D250D, AtzA mutant, substrate concentration 153 microM, enzyme concentration 10 nM, measuring absorbance of atrazine after reaction with pyridine at 436 nm |
690560 |