EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
3.7.1.8 | -999 |
- |
more |
in the steady-state hydrolysis of HOPDA, kcat/Km values are independent of methanol concentration, while both kcat and Km values increase with methanol concentration, stopped-flow spectrophotometry and steady-state kinetics, overview |
718941 |
3.7.1.8 | -999 |
- |
more |
Michaelis-Menten steady-state kinetics, stopped-flow measurements |
733185 |
3.7.1.8 | -999 |
- |
more |
pre-steady-state kinetic burst of BphD |
719664 |
3.7.1.8 | -999 |
- |
more |
values between 0.00013 and 0.00106 mM for processing of the 6-acryl-2-hydroxy-6-ketohexa-2,4-dienoic acid substrates |
676272 |
3.7.1.8 | 0.00013 |
- |
(2E,4E)-2-hydroxy-6-(4-chlorophenyl)-6-oxohexa-2,4-dienoic acid |
- |
674405 |
3.7.1.8 | 0.00019 |
- |
(2E,4E)-2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid |
not chlorinated |
674405 |
3.7.1.8 | 0.0002 |
- |
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid |
pH 7.5, 25°C, recombinant wild-type BphD, without methanol |
718941 |
3.7.1.8 | 0.00028 |
- |
2,6-dioxo-6-phenylhexa-3-enoate |
pH 7.0, 25°C, mutant M148D |
733185 |
3.7.1.8 | 0.0003 |
- |
2-Hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid |
wild-type at 3.2°C |
672058 |
3.7.1.8 | 0.00033 |
- |
(2E,4E)-6-(2-chlorophenyl)-2-hydroxy-6-oxohexa-2,4-dienoic acid |
- |
674405 |