EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
3.4.21.50 | 0.0007 |
- |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin |
pH 9.0, 37°C, mutant enzyme H210S |
651790 |
3.4.21.50 | 0.0013 |
- |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin |
pH 9.0, 37°C, mutant enzyme H210A |
651790 |
3.4.21.50 | 0.0019 |
- |
Trypsinogen |
- |
36606, 36618 |
3.4.21.50 | 0.0021 |
- |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin |
pH 9.0, 37°C, wild-type enzyme |
651790 |
3.4.21.50 | 0.0028 |
- |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin |
pH 9.0, 37°C, wild-type enzyme |
651790 |
3.4.21.50 | 0.0034 |
- |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin |
pH 9.0, 37°C, mutant enzyme W169L |
651790 |
3.4.21.50 | 0.0036 |
- |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin |
pH 9.0, 37°C, mutant enzyme W169F |
651790 |
3.4.21.50 | 0.0037 |
- |
tert-butyloxycarbonyl-Ala-Lys-7-amido-4-methylcoumarin |
pH 9.0, 37°C, wild-type enzyme |
651790 |
3.4.21.50 | 0.004 |
- |
tert-butyloxycarbonyl-Ala-Ala-Lys-7-amido-4-methylcoumarin |
pH 9.0, 37°C, mutant enzyme W169F |
651790 |
3.4.21.50 | 0.0043 |
- |
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin |
pH 9.0, 37°C, mutant enzyme W169Y |
651790 |