EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    3.2.1.22 | -999 |
- |
more |
hydrolysis of alpha-galactosides with poor leaving groups is Michaelian, but that of substrates with good leaving groups exhibits pronounced apparent substrate inhibition, with Kis values similar to Km values |
741846 |
| 3.2.1.22 | 0.00135 |
- |
melibiose |
- |
696818 |
| 3.2.1.22 | 0.03 |
- |
alpha-D-galactosyl fluoride |
temperature not specified in the publication, pH 5 |
741846 |
| 3.2.1.22 | 0.037 |
- |
2,4-dinitrophenyl alpha-D-galactoside |
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C |
678262 |
| 3.2.1.22 | 0.039 |
- |
beta-naphthyl alpha-D-galactoside |
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C |
678262 |
| 3.2.1.22 | 0.04 |
- |
4-nitrophenyl alpha-D-galactopyranoside |
pH 5.0, 65°C, wild-type enzyme |
747913 |
| 3.2.1.22 | 0.048 |
- |
4-O-methylphenyl alpha-D-galactoside |
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C |
678262 |
| 3.2.1.22 | 0.05 |
- |
ceramide trihexoside |
- |
136492 |
| 3.2.1.22 | 0.075 |
- |
p-nitrophenyl alpha-D-galactopyranoside |
pH 5, 75°C |
656702 |
| 3.2.1.22 | 0.08 |
- |
4-nitrophenyl alpha-D-galactoside |
wild type enzyme, in 200 mM sodium citrate buffer (pH 5.0), at 37°C |
678262 |
