EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
3.1.4.39 | -999 |
- |
more |
affinity for lysophosphatidylcholine is significantly higher than for nucleotides |
696397 |
3.1.4.39 | -999 |
- |
more |
capable of hydrolyzing sphingosylphosphorylcholine with a significantly higher km-value, also able to hydrolyze nucleotide triphosphates di-adenosine polyphosphates and artificial phosphodiester substrates such as p-nitrophenyl thymidine phosphate with Km-values close to 1 and optimal activity observed at alkaline pH |
697490 |
3.1.4.39 | -999 |
- |
more |
steady-state, single-turnover, and transient kinetics, kinetic analysis and modeling, using artificial fluorescent substrate FS-3, overview |
715631 |
3.1.4.39 | -999 |
- |
more |
suggestion that lysoPLD predominantly utilizes saturated forms of lysophosphatidylcholine |
678393 |
3.1.4.39 | 0.0026 |
- |
FS-3 |
wild type enzyme, in 50 mM Tris pH 7.4, 5 mM CaCl2, 1 mg/ml bovine serum albumin, at 37°C |
705456 |
3.1.4.39 | 0.0031 |
- |
FS-2 |
- |
666527 |
3.1.4.39 | 0.0037 |
- |
FS-3 |
- |
702693 |
3.1.4.39 | 0.004 |
- |
coumarin-phosphate-fluorescein |
coumarin-phosphate-fluorescein is a fluorescence resonance energy transfer (FRET)-based sensor of phosphodiesterase activity. Very useful substrate for enzymatic analysis of purified autotaxin/lysoPLD and can also be used to detect autotaxin/lysoPLD activity in serum-free conditioned culture medium isolated from cells expressing the enzyme |
681769 |
3.1.4.39 | 0.004 |
- |
[5-[[(23S)-33-(4-[(E)-[4-(dimethylamino)phenyl]diazenyl]phenyl)-20,23-dihydroxy-20-oxido-15,26,33-trioxo-3,6,9,12,19,21,25-heptaoxa-16,32-diaza-20-phosphatritriacont-1-yl]carbamoyl]-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)phenyl]acetic acid |
[5-[[(23S)-33-(4-[(E)-[4-(dimethylamino)phenyl]diazenyl]phenyl)-20,23-dihydroxy-20-oxido-15,26,33-trioxo-3,6,9,12,19,21,25-heptaoxa-16,32-diaza-20-phosphatritriacont-1-yl]carbamoyl]-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)phenyl]acetic acid can be used to detect autotaxin/lysoPLD activity in human and mouse blood |
681769 |
3.1.4.39 | 0.0063 |
- |
5-[[(23R)-33-(4-[[4-(dimethylamino)phenyl]diazenyl]phenyl)-20,23-dihydroxy-20-oxido-15,33-dioxo-3,6,9,12,19,21,25-heptaoxa-16,32-diaza-20-phosphatritriacont-1-yl]carbamoyl]-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid |
- |
666527 |