EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
3.1.3.32 | 0.0000179 |
- |
3'-phospho-5'-hydroxy-DNA |
at pH 7.5 and 37°C |
730000 |
3.1.3.32 | 0.0039 |
- |
DNA with 5'-hydroxyl ends |
- |
81073 |
3.1.3.32 | 0.006 |
- |
3'-TMP |
- |
659503 |
3.1.3.32 | 0.069 |
- |
ATP |
- |
81073 |
3.1.3.32 | 0.548 |
- |
3'-phosphopolynucleotide |
- |
81073 |
3.1.3.32 | 0.7 |
- |
cAMP |
- |
659503 |
3.1.3.32 | 1 |
- |
p-nitrophenyl phosphate |
mutant enzyme H376N, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2 |
674594 |
3.1.3.32 | 1.3 |
- |
p-nitrophenyl phosphate |
mutant enzyme D392E, at 45°C, in 50 mM Tris-HCl (pH 7.5), 0.5 mM MnCl2 |
674594 |
3.1.3.32 | 1.6 |
- |
bis-p-nitrophenyl phosphate |
mutant enzyme D236A, at 45°C, in 50 mM Tris-HCl (pH 8.0) 0.5 mM NiCl2 |
674594 |
3.1.3.32 | 2.2 |
- |
bis-p-nitrophenyl phosphate |
mutant enzyme D236N, at 45°C, in 50 mM Tris-HCl (pH 8.0) 0.5 mM NiCl2 |
674594 |