EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   3.1.1.72 | -999 |
- |
more |
1.42 mg/mL with glycol chitin as substrate, in 50 mM Tris-HCl buffer, pH 7.0, at 60°C for 20 min |
708516 |
| 3.1.1.72 | 0.00186 |
- |
p-nitrophenyl acetate |
30°C, pH 8.0 |
697655 |
| 3.1.1.72 | 0.04 |
- |
4-nitrophenyl acetate |
fusion protein with CBM3, pH 7.5, 50°C |
749963 |
| 3.1.1.72 | 0.05 |
- |
4-nitrophenyl acetate |
wild-type, pH 7.5, 50°C |
749963 |
| 3.1.1.72 | 0.056 |
- |
Alpha-naphthyl acetate |
pH 6.5, 37°C, acetylxylan esterase Axe6B |
664492 |
| 3.1.1.72 | 0.07 |
- |
tetraacetyl-xylopyranoside |
mutant TM2, at 37°C, in 100 mM Tris-HCl buffer, pH 7.4, and 3 mM MgCl2 |
708940 |
| 3.1.1.72 | 0.079 |
- |
4-methylumbelliferyl acetate |
mutant carrying a replacement of CBM1 with CBM6, pH 8.0, 50°C |
750189 |
| 3.1.1.72 | 0.08 |
- |
tetraacetyl-xylopyranoside |
wild-type, at 37°C, in 100 mM Tris-HCl buffer, pH 7.4, and 3 mM MgCl2 |
708940 |
| 3.1.1.72 | 0.084 |
- |
Alpha-naphthyl acetate |
pH 6.5, 37°C, acetylxylan esterase Axe6A |
664492 |
| 3.1.1.72 | 0.087 |
- |
4-methylumbelliferyl acetate |
wild-type lacking CBM1, 50°C, pH 8.0 |
750189 |
