EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
2.5.1.2 | 0.003 |
- |
thiamine |
measured in Pi-Tris and Pi-diphosphate, the same as for thiamine diphosphate |
636851 |
2.5.1.2 | 0.0037 |
- |
thiamine |
- |
636853 |
2.5.1.2 | 0.0087 |
- |
thiamine |
pH 5.8, 25°C, the enzyme does not follow strict Michaelis-Menten kinetics |
636846 |
2.5.1.2 | 0.0211 |
- |
thiamine |
- |
684401 |
2.5.1.2 | 0.0211 |
- |
thiamine |
pH 7.2, temperature not specified in the publication |
684401 |
2.5.1.2 | 0.03 |
- |
thiamine |
mutant D302N, pH 8.0, 37°C |
737686 |
2.5.1.2 | 0.0364 |
- |
4-nitrothiophenolate |
- |
684401 |
2.5.1.2 | 0.0364 |
- |
4-nitrothiophenolate |
pH 7.2, temperature not specified in the publication |
684401 |
2.5.1.2 | 0.075 |
- |
thiamine |
mutant E271Q, pH 8.0, 37°C |
737686 |
2.5.1.2 | 0.25 |
- |
thiamine |
mutant Y46F, pH 8.0, 37°C |
737686 |