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EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Commentary	Reference
1.3.8.1	-999	-	more	the tight non-dissociating bifurcating Etf-Bcd complex of Closstridium difficile exhibits normal Michaelis-Menten kinetics for NADH	742866
1.3.8.1	0.0003	-	butanoyl-CoA	32°C, pH 7.0	654618
1.3.8.1	0.00045	-	butanoyl-CoA	32°C, pH 7.0, R147W mutant	654618
1.3.8.1	0.0006	-	butyryl-CoA	-	391309
1.3.8.1	0.0006	-	butyryl-CoA	wild-type enzyme	391307
1.3.8.1	0.00075	-	butanoyl-CoA	32°C, pH 7.0, G185S mutant	654618
1.3.8.1	0.00083	-	acrylyl-CoA	22°C, pH 6.4	696519
1.3.8.1	0.0011	-	octanoyl-CoA	E368G/G247E double mutant enzyme	391307
1.3.8.1	0.0015	-	butyryl-CoA	E368D mutant enzyme	391307
1.3.8.1	0.0017	-	hexanoyl-CoA	E368G/G247E double mutant enzyme	391307

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Release 2023.1 (January 2023)