EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    1.3.1.95 | 0.00083 |
- |
acryloyl-CoA |
in 0.1 M phosphate buffer pH 6.4, at 22°C |
696519 |
| 1.3.1.95 | 0.002 |
- |
acryloyl-CoA |
apparent value, in 50 mM Tris/HCl, pH 7.0, at 25°C |
697707 |
| 1.3.1.95 | 0.008 |
- |
(E)-2-butenoyl-CoA |
in 0.1 M phosphate buffer pH 6.4, at 22°C |
696519 |
| 1.3.1.95 | 0.008 |
- |
NADH |
using acryloyl-CoA as cosubstrate, in 50 mM Tris/HCl, pH 7.0, at 25°C |
697707 |
| 1.3.1.95 | 0.01 |
- |
acryloyl-CoA |
apparent value, Km less than 0.01 mM, recombinant enzyme, at pH 7.5 and 22°C |
698624 |
| 1.3.1.95 | 0.036 |
- |
acryloyl-CoA |
apparent value, recombinant enzyme, at pH 7.5 and 22°C |
698624 |
| 1.3.1.95 | 0.036 |
- |
NADPH |
apparent value, recombinant enzyme, at pH 7.5 and 22°C |
698624 |
| 1.3.1.95 | 0.77 |
- |
ethyl vinyl ketone |
in 0.1 M phosphate buffer pH 6.4, at 22°C |
696519 |
| 1.3.1.95 | 1.8 |
- |
3-buten-2-one |
apparent value, in 50 mM Tris/HCl, pH 7.0, at 25°C |
697707 |
| 1.3.1.95 | 5.6 |
- |
acrolein |
in 0.1 M phosphate buffer pH 6.4, at 22°C |
696519 |
