EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
1.2.4.1 | -999 |
- |
more |
detailed kinetic and thermodynamic analysis |
672086 |
1.2.4.1 | -999 |
- |
more |
kinetics |
655474 |
1.2.4.1 | -999 |
- |
more |
sigmoidal dependence on the concentration of pyruvate, Hill-coefficient: 1.85 |
348960 |
1.2.4.1 | -999 |
- |
more |
with aging of rats the Vmax of the enzyme complex increases by 60%, while the Km decreases by 1.6fold in the same time, enzyme expression level remaines constant, more efficient catabolization of pyruvate due to adaptive phosphorylation of the enzyme within the multienzyme complex, age-related decline in pyruvate dehydrogenase kinase PDK-4 |
654462 |
1.2.4.1 | 0.00056 |
- |
thiamine diphosphate |
mutant R267A, pH 7.0, 25°C |
655474 |
1.2.4.1 | 0.0009 |
- |
thiamine diphosphate |
recombinant enzyme |
348987 |
1.2.4.1 | 0.0011 |
- |
pyruvate |
wild-type enzyme, pH 7.0, 25°C |
655474 |
1.2.4.1 | 0.0011 |
- |
pyruvate |
wild-type, pH 7.0, 30°C |
735664 |
1.2.4.1 | 0.0012 |
- |
pyruvate |
mutant F266A, pH 7.0, 25°C |
655474 |
1.2.4.1 | 0.0015 |
- |
thiamine diphosphate |
recombinant enzyme |
348987 |