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EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Commentary	Reference
1.2.1.27	-999	-	more	Km-value of CoA for R301L mutant enzyme under steady-state conditions with propionaldehyde is above 1 mM at pH 8.2 and 30°C	725429
1.2.1.27	0.0025	-	2-Methyl-3-oxopropanoate	-	390223
1.2.1.27	0.0045	-	malonate semialdehyde	-	288055, 390223
1.2.1.27	0.0053	-	2-Methyl-3-oxopropanoate	-	288055
1.2.1.27	0.006	-	methylmalonate semialdehyde	R124L mutant enzyme, steady-state conditions, pH 8.2 and 30°C	725429
1.2.1.27	0.019	-	2-Methyl-3-oxopropanoate	-	390219
1.2.1.27	0.021	-	CoA	-	390218
1.2.1.27	0.021	-	methylmalonate semialdehyde	V229G/H226P/G225 insertion, pH 8.2 and 30°C	725485
1.2.1.27	0.022	-	CoA	-	390219
1.2.1.27	0.022	-	methylmalonate semialdehyde	G225 insertion, pH 8.2 and 30°C	725485

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Release 2023.1 (January 2023)