EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    1.14.13.1 | -999 |
- |
more |
- |
390002, 390004, 390005, 390006, 439423 |
| 1.14.13.1 | -999 |
- |
more |
FAD, salicylate and NADH, comparison of wild-type, recombinant and mutant enzyme |
390014 |
| 1.14.13.1 | -999 |
- |
more |
kinetic analysis, rate-limiting steps, detailed overview |
764872 |
| 1.14.13.1 | -999 |
- |
more |
the kinetic profile of ShyA, obtained while only varying the salicylic acid concentration at a constant 1 mM concentration of the second substrate (NADH), does not follow Michaelis-Menten kinetics. ShyA reveals a sigmoidal curve. This may be caused by inhibition or inactivation due to low salicylate concentrations and relatively high NADH concentrations |
763980 |
| 1.14.13.1 | 0.0005 |
- |
salicylate |
enzyme form NahG |
675817 |
| 1.14.13.1 | 0.0006 |
- |
5-chlorosalicylate |
pH not specified in the publication, 25°C |
680483 |
| 1.14.13.1 | 0.0011 |
- |
salicylate |
30°C, pH 7.0 |
684569 |
| 1.14.13.1 | 0.0013 |
- |
salicylate |
at pH 7.5, temperature not specified in the publication |
741666 |
| 1.14.13.1 | 0.0016 |
- |
salicylate |
cosubstrate NADH |
390002 |
| 1.14.13.1 | 0.0016 |
- |
salicylate |
native enzyme |
390013 |
