EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
   1.13.12.6 | -999 |
- |
more |
Michaelis-Menten kinetics |
744465, 744466, 746376 |
| 1.13.12.6 | 0.00028 |
- |
Cypridina luciferin |
recombinant enzyme, pH and temperature not specified in the publication |
746376 |
| 1.13.12.6 | 0.00045 |
- |
Vargula hilgendorfii luciferin |
recombinant enzyme, recombinant substrate, pH and temperature not specified in the publication |
746376 |
| 1.13.12.6 | 0.0005 |
- |
Luciferin |
- |
439330 |
| 1.13.12.6 | 0.00052 |
- |
Vargula hilgendorfii luciferin |
recombinant enzyme, native substrate, pH and temperature not specified in the publication |
746376 |
| 1.13.12.6 | 0.0016 |
- |
Cypridina luciferin |
35 nM recombinant prostaglandin E2-luciferase diluted with 0.1 M Tris buffer (pH 7.4) containing 0.05% bovine serum albumin, 1 nM monoclonal anti-prostaglandin E2 antibody, incubation for 18 h, 4°C, in the dark, addition of 1 microM Cypridina luciferin in 0.1 M Tris-HCl buffer (pH 7.4) containing 0.3 M sodium ascorbate and 0.02 M Na2SO3 for bioluminescence reading |
699954 |
| 1.13.12.6 | 0.03 |
- |
1-{3-[6-(1H-indol-3-yl)-2-(1-methylpropyl)-3-oxo-3,7-dihydroimidazo[1,2-a]pyrazin-8-yl]propyl}guanidine |
pH 7.4 |
655065 |
