EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
1.13.12.22 | 0.068 |
- |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate |
pH 7.0, 22°C, mutant enzyme N63A |
729205 |
1.13.12.22 | 0.094 |
- |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate |
pH 7.0, 22°C, wild-type enzyme, recombinant enzyme with N-terminal polyhistidine tag |
729205 |
1.13.12.22 | 0.1 |
- |
dithranol |
at pH 9.8 and 20°C |
742892 |
1.13.12.22 | 0.12 |
- |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate |
pH 7.0, 22°C, mutant enzyme N18A |
729205 |
1.13.12.22 | 0.133 |
- |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate |
pH 7.0, 22°C, mutant enzyme R90E |
729205 |
1.13.12.22 | 0.14 |
- |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate |
pH 7.0, 22°C, wild-type enzyme, recombinant enzyme construct with a His tag at the C-terminus |
729205 |
1.13.12.22 | 0.15 |
- |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate |
pH 7.0, 22°C, mutant enzyme H49A |
729205 |
1.13.12.22 | 0.22 |
- |
dithranol |
at pH 6.3 and 20°C |
742892 |
1.13.12.22 | 0.22 |
- |
[4,5-dihydroxy-10-oxo-3-(3-oxobutanoyl)-9,10-dihydroanthracen-2-yl]acetate |
pH 7.0, 22°C, mutant enzyme W67F |
729205 |
1.13.12.22 | 0.41 |
- |
O2 |
at pH 9.8 and 20°C |
742892 |