EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
1.13.11.50 | -999 |
- |
more |
kinetic first-order rate constants of substrate ligation and steady-state kinetic studies of wild-type and mutant enzymes, overview |
711267 |
1.13.11.50 | -999 |
- |
more |
steady-state and single-turnover kinetics of substrate binding and conversion in wild-type Dke1 and mutants, overview |
725553 |
1.13.11.50 | 0.000009 |
- |
Pentane-2,4-dione |
- |
636327 |
1.13.11.50 | 0.002 |
- |
4,4-difluoro-1-phenyl-1,3-butanedione |
apparent value at 25°C |
674150 |
1.13.11.50 | 0.0025 |
- |
1,1,1-trifluoro-2,4-pentanedione |
apparent value at 25°C |
674150 |
1.13.11.50 | 0.0027 |
- |
1,1-difluoro-2,4-pentanedione |
apparent value at 25°C |
674150 |
1.13.11.50 | 0.0034 |
- |
Pentane-2,4-dione |
pH 7.5, 25°C |
657614 |
1.13.11.50 | 0.006 |
- |
Pentane-2,4-dione |
Strep-tagged enzyme, at pH 7.5 |
671683 |
1.13.11.50 | 0.006 |
- |
Pentane-2,4-dione |
wild type enzyme, using 0.2 mM pentane-2,4-dione in 20 mM Tris/HCl buffer at pH 7.5 and 25°C |
675466 |
1.13.11.50 | 0.0065 |
- |
Pentane-2,4-dione |
mutant enzyme E69Q, using 0.2 mM pentane-2,4-dione in 20 mM Tris/HCl buffer at pH 7.5 and 25°C |
675466 |