EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    1.1.99.31 | 0.04 |
- |
(S)-Mandelate |
pH 7.5, 20°C, mutant enzyme, G81A/MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach and a G81A mutation in MDH |
667577 |
| 1.1.99.31 | 0.04 |
- |
Mandelate |
mutant G81A of chimera MDH-GOX2 |
695329 |
| 1.1.99.31 | 0.04 |
- |
O2 |
pH 7.5, 20°C, mutant enzyme G81V |
667577 |
| 1.1.99.31 | 0.074 |
- |
(S)-3-phenyllactate |
pH 7.5, 20°C, mutant enzyme R277G |
667567 |
| 1.1.99.31 | 0.08 |
- |
3-hydroxy-D,L-mandelate |
pH 7.5, 25°C, cosubstrate: ferricyanide |
667457 |
| 1.1.99.31 | 0.085 |
- |
(S)-Mandelate |
pH 7.5, 20°C, cosubstrate: 2,6-dichloroindophenol, MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach |
667565 |
| 1.1.99.31 | 0.09 |
- |
(S)-Mandelate |
pH 7.5, 20°C, mutant enzyme MDH-GOX2, a chimeric mutant of (S)-mandelate dehydrogenase with residues 177-215 replaced by residues 176-195 of glycolate oxidase from spinach |
667577 |
| 1.1.99.31 | 0.09 |
- |
Mandelate |
chimera MDH-GOX2 |
695329 |
| 1.1.99.31 | 0.11 |
- |
(S)-Mandelate |
pH 7.5, 4°C |
667575 |
| 1.1.99.31 | 0.12 |
- |
(S)-Mandelate |
pH 7.5, 20°C |
667575 |
